Instituição onde foi realizado o trabalho
- Principal: Instituto de Ensino e Pesquisa Santa Cruz
- Secundaria: Universidade de São Paulo (USP)
- MARIANA AKEMI MATSURA MISAWA (Interesse Comercial: NÃO)
- Tatiana Tanaka (Interesse Comercial: NÃO)
- Tomás Minelli (Interesse Comercial: NÃO)
- Pedro Gomes Oliveira Braga (Interesse Comercial: NÃO)
- Juliana Mika Kato (Interesse Comercial: NÃO)
- Michele Soares Gomes Gouvêa (Interesse Comercial: NÃO)
- João Renato Rebello Pinho (Interesse Comercial: NÃO)
- Joyce Hisae Yamamoto (Interesse Comercial: NÃO)
RRT-PCR DETECTION OF SARS-COV-2 IN CONJUNCTIVAL SWABS FROM PATIENTS WITH SEVERE FORMS OF COVID-19
To test conjunctival swabs from patients with laboratory-confirmed severe forms of coronavirus disease 2019 (COVID-19) for the presence of SARS-CoV-2 on real-time reverse-transcription polymerase chain reaction (rRT-PCR).
Fifty conjunctival swabs were collected from 50 in-patients with laboratory-confirmed severe forms of COVID-19 at a teaching and referral hospital in São Paulo, Brazil. The samples were tested for SARS-CoV-2 on rRT-PCR and compared with naso/oropharyngeal swabs collected within 24 hours of the conjunctival swabs.
Five conjunctival samples (10%) tested positive (amplification of the N1 and N2 primer/probe sets) while two conjunctival samples (4%) yielded inconclusive results (amplification of the N1 primer/probe set only). The naso/oropharyngeal swabs were positive for SARS-CoV-2 on rRT-PCR in 34 patients (68%), negative in 14 (28%) and inconclusive in 2 (4%). The 5 patients with positive conjunctival swabs had positive (n=2), negative (n=2) or inconclusive (n=1) naso/oropharyngeal swabs on rRT-PCR. Patients with negative or inconclusive naso/oropharyngeal swabs had the diagnosis of COVID-19 confirmed by previous positive rRT-PCR results or by serology.
Sars-CoV-2 was detected in the conjunctival swabs of 10% of 50 patients with severe forms of COVID-19, reinforcing the notion that viral loads in the conjunctiva are higher in severe disease. The eye is a possible source of contagion, especially in severe forms of COVID-19.